𝐂𝐨𝐧𝐟𝐢𝐫𝐦𝐚𝐭𝐨𝐫𝐲 𝐓𝐞𝐬𝐭 𝐅𝐨𝐫 𝐁𝐥𝐨𝐨𝐝

𝐂𝐨𝐧𝐟𝐢𝐫𝐦𝐚𝐭𝐨𝐫𝐲 𝐓𝐞𝐬𝐭 𝐅𝐨𝐫 𝐁𝐥𝐨𝐨𝐝

Microcrystal Test

• In microcrystal test, chemicals are applied to dried blood stains which target the non-protein heme group of hemoglobin, the oxygen carrying protein of RBCs that belongs to class of compound known as porphyrins.
• These assays cannot distinguish between human and animal blood.

Teichmann’s test

• Also known as Hematin Crystal Assay.
• Teichmann’s reagent:

1. KCl, KBr and KI 0.1 g each
2. Glacial Acetic Acid 100ml

Procedure:

• Suspected blood sample is placed on a microscopic slide.
• Then glacial acetic acid is added to the slide with small amount of chloride which is followed by heating.
• As a result, crystals of ferriprotoporphyrin chloride are formed.
• Crystals are rhombic in shape and are brown in colour.
  
  
  

Takayama Test

• It is also known as Haemochromogen crystal assay test.
• Takayama's reagent: 

1. Saturated solution of glucose 3 ml
2. Pyridine solution 3 ml
3. 10% Sodium Hydroxide Solution 3 ml
4. Glacial acetic acid 7 ml

Procedure:

• Heme is gently heated under alkaline conditions in the presence of a reducing sugar such as glucose.
• Pink needle shaped crystals of pyridine ferriprotoporphyrin or Haemochromogen are formed.

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